Human fungal pathogen research

In the laboratory several PhD students and postdocs are working on Candida albicans and Candida glabrata, the two most common human fungal pathogen Candida species.  Our group focuses on in vitro and in vivo biofilm formation, the latter one in a subcutaneous rodent model system. We are also mainly interested in how these cells can sense where they are in the host. We mainly focus on the sensing of sugars and amino acids, with a specific focus on methionine as this amino acid is important for yeast-to-hyphae transition, an important virulence factor in C. albicans. We are also identifying the molecular mechanisms why the azoles, the most used class of antifungals are only fungistatic whereas their cellular target is an essential enzyme. We identified a novel mechanism of tolerance, which we are currently characterizing.  Finally, we are also developing novel tools to be used in Candida species. We are generating the first genome-wide two-hybrid library to identify specific protein-protein interactions in C. albicans, that then may be targeted by small molecules in an effort to identify novel antifungal drugs. We are also developing FRET-based on BiFC-based biosensors to determine activity of certain pathways, e.g. the cAMP-PKA pathway. protein-protein interactions using confocal microscopy. Depending on the interest of the applicant, he or she can be enrolled in one of these topics. Guidance will be done by a PhD student or postdoc.

• Projects are available in the Fall and Spring semester.
• Number of places available: 1 per semester.